Search results for "Restriction Site Polymorphism"

showing 3 items of 3 documents

Genetic differentiation in the striped dolphin Stenella coeruleoalba from European waters according to mitochondrial DNA (mtDNA) restriction analysis

1999

We used mitochondrial DNA (mtDNA) restriction analysis to study genetic variation in 98 striped dolphins (Stenella coeruleoalba) stranded on coasts from different European countries and from animals caught by fisheries. A total of 63 different restriction sites was mapped after digestion of mtDNA with 15 restriction endonucleases that yielded a total of 27 haplotypes. No haplotype was shared between Mediterranean and Atlantic areas. All the analyses indicate the existence of two different populations with a very limited gene flow across the Strait of Gibraltar.

Geneticseducation.field_of_studyMitochondrial DNADolphinsRestriction MappingPopulationGenetic VariationZoologyStenella coeruleoalbaBiologyDNA MitochondrialEuropeRestriction Site PolymorphismRestriction siteRestriction enzymeGenetics PopulationRestriction mapHaplotypesbiology.animalGenetic variationGeneticsAnimalseducationPhylogenyEcology Evolution Behavior and SystematicsMolecular Ecology
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Population structure and mitochondrial DNA gene flow in Old World populations of Drosophila subobscura

1992

An extensive survey of mitochondrial DNA (mtDNA) restriction polymorphism in 156 isofemale lines from 29 different geographic populations of Drosophila subobscura distributed throughout the Old World was carried out. Ten restriction enzymes were used, five of which revealed restriction site polymorphism. Of the 31 restriction sites detected, 13 were found to be polymorphic. Comparisons with the mtDNA map of Drosophila yakuba indicate that the variable sites are mainly concentrated in protein genes, especially those corresponding to the NADH complex. A total of 13 different haplotypes were observed, two of which (haplotypes I and II) are quite frequent and widely distributed throughout the p…

HeterozygoteMitochondrial DNAPopulationPopulation geneticsDNA MitochondrialGene FrequencyGeneticsAnimalseducationAllelesGenetics (clinical)Geneticseducation.field_of_studybiologybiology.organism_classificationDrosophila subobscuraIsoenzymesRestriction Site PolymorphismRestriction siteGenetics PopulationHaplotypesDrosophilaFemaleRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthDrosophila yakubaHeredity
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Mitochondrial DNA variability of striped dolphins (Stenella coeruleoalba) in the Spanish Mediterranean waters

1995

Frozen muscle samples from 44 striped dolphins stranded on the Spanish Mediterranean coasts from 1990 to 1993 have been studied by means of mitochondrial DNA (mtDNA) restriction site analysis. Thirty-five of these dolphins were affected by a die-off occurring during this time in the western Mediterranean Sea. The mtDNA from each dolphin was digested with 15 restriction endonucleases that recognized 61 different restriction sites. The specific location of these sites on the mitochondrial gene map allowed us to determine the distribution of variability along this molecule. From the restriction analysis, a total of 15 different composite patterns or haplotypes was obtained and their phylogenet…

Mitochondrial DNAeducation.field_of_studyEcologyPopulationZoologyCetaceaStenella coeruleoalbaAquatic ScienceBiologybiology.organism_classificationRestriction Site PolymorphismRestriction siteRestriction enzymebiology.animalRestriction fragment length polymorphismeducationhuman activitiesEcology Evolution Behavior and Systematics
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